MICROBIOLOGY EXAM #2
Understand and be able to interpret a bacterial growth curve : what do each of the different phases tell us about the physiological state of the bacteria.
What is generation time?
How is it obtained?
What is meant by exponential growth?
What is meant by binary fission?
How do environmental factors affect cell growth and why?
Why are some bacteria anaerobic? What do they lack? Why is this important?
Know the various descriptions of bacteria based on their response to environmental factors:
Be able to label and describe the various stages in a growth curve
Be able to determine from a growth curve the generation time of a bacterium.
Be able to describe the various ways by which microbes can be counted.
GENETICS and BIOTECHNOLOGY
Know the differences between DNA and RNA.
Know what happens in transcription
Know what happens in translation
Be able to explain (and compare) the workings of an inducible operon and a repressible operon
Explain the concept of catabolite repression
Know the different types of extra-chromosomal DNA and what each does
Know how DNA can enter a cell and be changed during its replication
Know the different types of mutations and how they might affect the cell
Know the ways in which humans can manipulate DNA : cloning, recombination, PCR
What is a virus?
Explain the generalized life cycle of a virus
Explain the differences between the various types of viruses based on nucleic acid composition
Be able to give a sample description of a various viral diseases and their causative agents
Know the general characteristics that define the protists ( esp. the protozoans)
Know the characteristics of the protist diseases discussed in class:
African sleeping sickness
Know the name of the disease, the area affected and the entire name of the pathogen
Dermatomycoses (dermatophytes) : tineas, blastomycosis, candidiasis
Respiratory mycoses: histoplasmosis, coccidiodomycosis
Helminthic diseases :
Be familiar with general characteristics of the helminthes described in class: where found and the diseases they cause